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Molecular Biology Insights Oligo v7.54 引物設計軟體 英文破解版

　


　
軟體簡介:
　
OLIGO 是一個從序列檔中搜索和選擇寡核?酸的多功能程式，應用于聚合鏈反應
(PCR),DNA測序，定點突變及各種各樣的雜交研究。
OLIGO根據最近鄰熱力學值計算寡核?酸的雜交溫度和二級結構。 這個好工具也用
於構建合成基因，在已合成的基因中發現適當的序列引物，發現和多元化一致的
引物和探針， 甚至發現蛋白質潛在的限制位點。

OlIGO 產品特色：
1. 分析TM和內部穩定性，繪製溶解溫度圖和穩定性圖；
2. 給出寡核?酸的重要相關資訊；
3. 顯示正向引物、反向引物和當前的OLIGO裏潛在的連接物。潛在的二聚物也可顯示出來；
4. 分析顯示在選中的正向引物或反向引物中基本的成份和溶解溫度；
5. 精確地分析出生物資訊軟體中的錯配位元點；
6. 正向引物和反向引物選中後，能自動產生PCR實驗條件和PCR產物資訊。

　
OLIGO Primer Analysis Software is the essential tool for designing and
analyzing sequencing and PCR primers, synthetic genes, and various kinds of
probes including siRNA and molecular beacons.
Based on the most up-to date nearest neighbor thermodynamic data, Oligo's
search algorithms find optimal primers for PCR, including TaqMan, highly
multiplexed, consensus or degenerate primers. Multiple file batch
processing is possible. It is also an invaluable tool for site directed
mutagenesis.
For each primer or primer pair, Oligo's various analysis windows show a
multitude of useful data, such as DNA and RNA secondary structure, dimer
formation, false priming and homology, internal stability, composition and
physical properties. With Oligo you can analyze open reading frames down to
predicted molecular weight and pKa of proteins, and search for restriction
enzyme sites, not only in DNA but also in reverse-translated proteins.
The first version of the software appeared on the market in 1989. It went
through several modifications, and the last one, the change from version 6
to 7, was the most comprehensive. Oligo 7 can automatically select multiplex
primers, process sequence files in batch modes, automatically design PCR
primers to cover multiple DNA regions in just one search and automatically
find primer/probe sets for real time PCR or finds nested primers sets. Oligo
search protocols (scoring system) may be customized in detail, so you may
optimize the results according to your specific needs.